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細(xì)胞中文名稱人胚腎細(xì)胞
細(xì)胞英文名稱293 Cells, low passage
物種人
細(xì)胞形態(tài)特征上皮樣;多角
細(xì)胞生長特性人胚腎細(xì)胞293Cells,lowpassage細(xì)胞 貼壁生長
細(xì)胞特種特性293細(xì)胞是人胚胎腎細(xì)胞用剪切過的腺病毒5 DNA轉(zhuǎn)化得到,它們包含并表達(dá)腺病毒5的早期區(qū)段1,包含并表達(dá)腺病毒5的早期區(qū)段1,能互補(bǔ)E1失活的腺病毒突變株和載體,使其生長;在DNA轉(zhuǎn)染檢測中它們是特別有效的受體細(xì)胞;對于大多數(shù)即使不是全部人腺病毒的生長和溶菌斑測試,它們也表現(xiàn)很好。這些細(xì)胞廣泛應(yīng)用于因缺失或代換E1序列引起的E1失活突變株和腺病毒載體的繁殖與滴定;因此它們被用來構(gòu)建腺病毒載體,用于構(gòu)建腺病毒載體使用低代數(shù)細(xì)胞,一般不超過40代,對于保持這株細(xì)胞的優(yōu)良特性,正確的細(xì)胞培養(yǎng)方法
培養(yǎng)基
血清
細(xì)胞傳代方法
細(xì)胞傳代情況P27
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測陰性
A32154甘草查爾酮人胚腎細(xì)胞293Cells,lowpassage細(xì)胞CLicochalcone C144506-14-9C21H22O420mg
A32155甘草素Liquiritigenin578-86-9C15H12O4256.2520mg
A32156異甘草素Isoliquiritigenin961-29-5C15H12O4256.2520mg
A32157水甘草堿Tabersonine4429-63-4C21H24N2O2336.4320mg
A32158鬼臼毒素Podophyllotoxin518-28-5C22H22O8414.4120mg
A32159鬼臼毒素-4-O-葡萄糖苷Podophyllotoxin-4-O-glucoside 16481-54-2C28H32O1310mg
A321604'-去甲基表鬼臼毒素4'-Demethylepipodophyllotoxin6559-91-7 C21H20O8400.3820mg
A32161葛根素Puerarin3681-99-0C21H20O10432.3820mg
A321623'-甲氧基葛根素3’- Methoxy Puerarin117047-07-120mg
A321634'-O-甲基葛根素"4'-O-Methylpuerarin
""92117-94-7
"C22H22O9430.4110mg
A321643'-羥基葛根素人胚腎細(xì)胞293Cells,lowpassage細(xì)胞3'-hydroxy Puerarin117076-54-520mg
A32165鉤吻堿Gelsemine509-15-9C20H22N2O2322.420mg
A32166鉤吻素子koumine1358-76-5C20H22N2O2306.40720mg
A32167高良姜素3,5,7-Trihydroxyflavone548-83-4 C15H10O5270.2420mg
A32168三尖杉堿Cephalotaxlen24316-19-6C18H21NO4315.3620mg
A32169高三尖杉酯堿Homoharringtonine26833-87-4C29H39NO9545.6220mg
A32170鉤藤堿 Rhynchophylline76-66-4C22H28N2O4384.4720mg
A32171去氫鉤藤堿Corynoxeine630-94-4C22H26N2O4 20mg
A32172異去氫鉤藤堿Isocorynoxeine51014-29-0C22H26N2O4382.4520mg
A32173異鉤藤堿Isorhychophylline6859-1-4C21H26N2O4370.44620mg
A32174根皮素Phloretin 60-82-2C15H14O5274.2720mg
A32175根皮苷Phloridzin 7061-54-3C21H24O10436.4120mg
A32176格列風(fēng)內(nèi)酯Griffonilide61371-55-9C8H8O4168.0420mg
A32177果糖beta-D-Fructopyranose7660-25-5C6H12O6180.1620mg
A32178古倫賓ColumbinC20H22O6358.3920mg
A32179瓜子金皂苷己Polygalasaponin F20mg
A32180枸櫞酸Citric acid monohydrate5949-29-1C6H10O8210.1420mg
A32181甘露糖D-Mannitol69-65-8C6H14O6182.1720mg
A32182甘油三油酸酯"Triolein
"122-32-7C57H104O6885.4320mg
A32183光翠雀堿Denudatine 26166-37-0 C22H33NO3343.520mg
A32184甘松新酮Nardosinone23720-80-123720-80-1250.320mg
A32185告達(dá)庭Caudatin38395-02-7C28H42O7490.63320mg
A32186橄欖苦苷Oleuropein32619-42-4C25H32O13540.5120mg
A32187厚樸酚Magnolol 528-43-8C18H18O2266.3320mg
A32188和厚樸酚Honokiol 35354-74-6C18H18O2266.3320mg
A32189黃杞苷Engeletin572-31-6C21H22O10434.3920mg
A32190胡黃連苷ⅠPicrosideⅠ27409-30-9C24H28O11492.4720mg
人胚腎細(xì)胞293Cells,lowpassage細(xì)胞A32191胡黃連苷ⅡPicroside Ⅱ39012-20-9C23H28O13512.4620mg
A32192胡黃連苷 III64461-95-6C25H30O13538.49910mg
A32193蒿甲醚Artemether71963-77-4C16H26O5298.3720mg