服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
小鼠神經(jīng)絲蛋白酶免試劑盒,(NF)ELISA檢測試劑盒 ,英文名:NF ELISA Kit ,英文縮寫:NF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠神經(jīng)絲蛋白ELISA檢測試劑盒,NF 檢測試劑盒, NF 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠神經(jīng)絲蛋白酶免試劑盒,(NF)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
線粒體甘油-3-磷酸酰基轉(zhuǎn)移酶(GPAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM)48T/96TY12385Rattus norvegicus (Rat)
中間α-球蛋白抑制因子H4(ITIH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inter Alpha-Globulin Inhibitor H4 (ITIH4)48T/96TY12386Homo sapiens (Human)
無孢蛋白(ASPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Asporin (ASPN)48T/96TY12387Homo sapiens (Human)
過氧化物酶體增殖物激活受體γ輔激活因子1α(PPARγC1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)48T/96TY12388Homo sapiens (Human)
Gremlin 1蛋白(GREM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gremlin 1 (GREM1)48T/96TY12389Homo sapiens (Human)
游離三碘甲狀腺原氨酸(fT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Free Triiodothyronine (fT3)48T/96TY12390General
骺蛋白聚糖(EPYC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Epiphycan (EPYC)48T/96TY12391Homo sapiens (Human)
生長激素2(GH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Hormone 2 (GH2)48T/96TY12392Homo sapiens (Human)
次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)48T/96TY12393Mus musculus (Mouse)
神經(jīng)纖維網(wǎng)蛋白1(NRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuropilin 1 (NRP1)48T/96TY12394Rattus norvegicus (Rat)
線粒體解偶聯(lián)蛋白2(UCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uncoupling Protein 2, Mitochondrial (UCP2)48T/96TY12395Homo sapiens (Human)
保護素(CD59)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protectin (CD59)48T/96TY12396Homo sapiens (Human)
吻素1(KISS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kisspeptin 1 (KISS1)48T/96TY12397Rattus norvegicus (Rat)
胰高血糖素樣肽2(GLP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucagon Like Peptide 2 (GLP2)48T/96TY12398Homo sapiens (Human)
Ⅰ型膠原α1(COL1α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 1 (COL1a1)48T/96TY12399Homo sapiens (Human)
Ⅺ型膠原α1(COL11α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type XI Alpha 1 (COL11a1)48T/96TY12400Homo sapiens (Human)
透明質(zhì)酸結(jié)合蛋白2(HABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hyaluronan Binding Protein 2 (HABP2)48T/96TY12401Homo sapiens (Human)
癌胚抗原相關(guān)細胞粘附分子7(CEACAM7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 7 (CEACAM7)48T/96TY12402Homo sapiens (Human)
纖維蛋白原相關(guān)蛋白1(FGL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Like Protein 1 (FGL1)48T/96TY12403Homo sapiens (Human)
脂解激活脂蛋白受體(LSR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipolysis Stimulated Lipoprotein Receptor (LSR)48T/96TY12404Homo sapiens (Human)
絲氨酸蛋白酶8(PRSS8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protease, Serine 8 (PRSS8)48T/96TY12405Homo sapiens (Human)
橋粒芯膠粘蛋白1(DSC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmocollin 1 (DSC1)48T/96TY12406Mus musculus (Mouse)
G蛋白偶聯(lián)受體37(GPR37)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for G Protein Coupled Receptor 37 (GPR37)48T/96TY12407Homo sapiens (Human)
轉(zhuǎn)鈷胺素蛋白Ⅰ(TCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transcobalamin I (TCN1)48T/96TY12408Homo sapiens (Human)
分層蛋白(SFN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stratifin (SFN)48T/96TY12409Homo sapiens (Human)
游離甲狀腺素(fT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Free Thyroxine (fT4)48T/96TY12410General
凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prothrombin Fragment 1+2 (F1+2)48T/96TY12411Sus scrofa; Porcine (Pig)
輕肽鐵蛋白(FTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ferritin, Light Polypeptide (FTL)48T/96TY12412Homo sapiens (Human)
肌球蛋白ⅤA(MYO5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin VA (MYO5A)48T/96TY12413Mus musculus (Mouse)
氨基乙二酸轉(zhuǎn)氨酶(AADAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aminoadipate Aminotransferase (AADAT)48T/96TY12414Homo sapiens (Human)
興奮性氨基酸轉(zhuǎn)運蛋白2(EAAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Excitatory Amino Acid Transporter 2 (EAAT2)48T/96TY12415Mus musculus (Mouse)
鈉/鉀離子轉(zhuǎn)運ATP酶α2肽(ATP1α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2)48T/96TY12416Mus musculus (Mouse)
鈉/鉀離子轉(zhuǎn)運ATP酶β1肽(ATP1β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)48T/96TY12417Mus musculus (Mouse)
鈣離子轉(zhuǎn)運ATP酶B2(ATP2B2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2)48T/96TY12418Mus musculus (Mouse)
泛醌細胞色素C還原酶核心蛋白Ⅱ(UQCRC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2)48T/96TY12419Mus musculus (Mouse)
RalA結(jié)合蛋白1(RALBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RalA Binding Protein 1 (RALBP1)48T/96TY12420Homo sapiens (Human)
肌動蛋白相關(guān)蛋白2/3復(fù)合體亞基4(ARPC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actin Related Protein 2/3 Complex Subunit 4 (ARPC4)48T/96TY12421Mus musculus (Mouse)
神經(jīng)元正五聚蛋白Ⅰ(NPTX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuronal Pentraxin I (NPTX1)48T/96TY12422Homo sapiens (Human)
神經(jīng)元正五聚蛋白Ⅰ(NPTX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuronal Pentraxin I (NPTX1)48T/96TY12423Rattus norvegicus (Rat)
尿皮質(zhì)素2(UCN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 2 (UCN2)48T/96TY12424Mus musculus (Mouse)
解整合素金屬蛋白酶28(ADAM28)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 28 (ADAM28)48T/96TY12425Homo sapiens (Human)
尿皮質(zhì)素3(UCN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 3 (UCN3)48T/96TY12426Mus musculus (Mouse)
半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cystatin B (CSTB)48T/96TY12427Homo sapiens (Human)
信號素7A(SEMA7A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Semaphorin 7A (SEMA7A)48T/96TY12428Homo sapiens (Human)
肌肽酶1(CNDP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnosine Dipeptidase 1 (CNDP1)48T/96TY12429Homo sapiens (Human)
泛素結(jié)合酶E2C(UBE2C)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin Conjugating Enzyme E2C (UBE2C)48T/96TY12430Homo sapiens (Human)
Klotho蛋白(KL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Klotho (KL)48T/96TY12431Mus musculus (Mouse)
VEGF共調(diào)節(jié)趨化因子1(VCC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for VEGF Co Regulated Chemokine 1 (VCC1)48T/96TY12432Homo sapiens (Human)
VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for VGF Nerve Growth Factor Inducible (VGF)48T/96TY12433Rattus norvegicus (Rat)
細胞因子受體樣因子1(CRLF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytokine Receptor Like Factor 1 (CRLF1)48T/96TY12434Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。