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小鼠瘦素酶免試劑盒,(LEP)ELISA檢測試劑盒,英文名:LEP ELISA Kit,英文縮寫:LEP ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠瘦素ELISA檢測試劑盒,LEP 檢測試劑盒,LEP 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠瘦素酶免試劑盒,(LEP)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4)48T/96TY11285Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白1(IGFBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)48T/96TY11286Sus scrofa; Porcine (Pig)
免疫球蛋白樣EGF樣域酪氨酸激酶1(Tie1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY11287Bos taurus; Bovine (Cattle)
免疫球蛋白樣EGF樣域酪氨酸激酶1(Tie1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY11288Danio rerio (Zebrafish)
反胰島素樣生長因子2(IGF2AS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2, Antisense (IGF2AS)48T/96TY11289Rattus norvegicus (Rat)
內(nèi)皮素受體B(ETRB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endothelin Receptor B (ETRB)48T/96TY11290Homo sapiens (Human)
葡萄糖苷酸酶β(GUSβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY11291Rattus norvegicus (Rat)
葡萄糖苷酸酶β(GUSβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY11292Homo sapiens (Human)
葡萄糖苷酸酶β(GUSβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY11293Mus musculus (Mouse)
β-1,4-半乳糖轉(zhuǎn)移酶1(β4GALT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-1,4-Galactosyltransferase 1 (b4GALT1)48T/96TY11294Rattus norvegicus (Rat)
三葉因子2(TFF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY11295Mus musculus (Mouse)
三葉因子2(TFF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY11296Rattus norvegicus (Rat)
葡萄糖激酶(GCK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucokinase (GCK)48T/96TY11297Mus musculus (Mouse)
葡萄糖激酶(GCK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucokinase (GCK)48T/96TY11298Rattus norvegicus (Rat)
主要堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Basic Protein (MBP)48T/96TY11299Homo sapiens (Human)
白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Albumin (ALB)48T/96TY11300Oryctolagus cuniculus (Rabbit)
血管性血友病因子(vWF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor (vWF)48T/96TY11301Canis familiaris; Canine (Dog)
白介素7(IL7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 7 (IL7)48T/96TY11302Homo sapiens (Human)
紅細(xì)胞生成素(EPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Erythropoietin (EPO)48T/96TY11303Sus scrofa; Porcine (Pig)
血小板內(nèi)皮細(xì)胞粘附分子1(PECAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet/Endothelial Cell Adhesion Molecule (PECAM1)48T/96TY11304Rattus norvegicus (Rat)
平滑肌肌動蛋白α2(ACTα2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actin Alpha 2, Smooth Muscle (ACTa2)48T/96TY11305Mus musculus (Mouse)
堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY11306Sus scrofa; Porcine (Pig)
軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Netrin 1 (Ntn1)48T/96TY11307Mus musculus (Mouse)
表面活性物質(zhì)關(guān)聯(lián)蛋白C(SPC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein C (SPC)48T/96TY11308Rattus norvegicus (Rat)
Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 2 (TLR2)48T/96TY11309Bos taurus; Bovine (Cattle)
Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 4 (TLR4)48T/96TY11310Bos taurus; Bovine (Cattle)
信號傳導(dǎo)轉(zhuǎn)錄激活因子4(STAT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 4 (STAT4)48T/96TY11311Canis familiaris; Canine (Dog)
血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY11312Mus musculus (Mouse)
血管緊張素轉(zhuǎn)化酶2(ACE2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)48T/96TY11313Homo sapiens (Human)
卵白蛋白(OVA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ovalbumin (OVA)48T/96TY11314General
基質(zhì)金屬蛋白酶16(MMP16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 16 (MMP16)48T/96TY11315Rattus norvegicus (Rat)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY11316Chicken (Gallus)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY11317Canis familiaris; Canine (Dog)
α-血紅蛋白穩(wěn)定蛋白(αHSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP)48T/96TY11318Rattus norvegicus (Rat)
紐蛋白(VCL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vinculin (VCL)48T/96TY11319Mus musculus (Mouse)
葡萄糖轉(zhuǎn)運蛋白4(GLUT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Transporter 4 (GLUT4)48T/96TY11320Rattus norvegicus (Rat)
癌胚抗原(CEA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen (CEA)48T/96TY11321Rattus norvegicus (Rat)
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4)48T/96TY11322Mus musculus (Mouse)
血管性血友病因子裂解蛋白酶(vWFcp)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP)48T/96TY11323Homo sapiens (Human)
催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolactin (PRL)48T/96TY11324Sus scrofa; Porcine (Pig)
神經(jīng)調(diào)節(jié)肽U受體1(NMUR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuromedin U Receptor 1 (NMUR1)48T/96TY11325Homo sapiens (Human)
八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4)48T/96TY11326Mus musculus (Mouse)
白介素4(IL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY11327Bos taurus; Bovine (Cattle)
基質(zhì)細(xì)胞衍生因子2(SDF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stromal Cell Derived Factor 2 (SDF2)48T/96TY11328Rattus norvegicus (Rat)
乳過氧化物酶(LPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoperoxidase (LPO)48T/96TY11329Rattus norvegicus (Rat)
乳過氧化物酶(LPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoperoxidase (LPO)48T/96TY11330Mus musculus (Mouse)
γ-谷氨酰轉(zhuǎn)移酶1(γGT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Gamma-Glutamyltransferase 1 (gGT1)48T/96TY11331Mus musculus (Mouse)
基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY11332Oryctolagus cuniculus (Rabbit)
蛋白C(PROC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein C (PROC)48T/96TY11333Sus scrofa; Porcine (Pig)
肝素結(jié)合性表皮生長因子(HBEGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)48T/96TY11334Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。