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人軍團菌抗原酶免試劑盒,(LP Ag)ELISA檢測試劑盒,英文名:LP Ag ELISA Kit,英文縮寫:LP Ag ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人軍團菌抗原ELISA檢測試劑盒,LP Ag 檢測試劑盒,LP Ag 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人軍團菌抗原酶免試劑盒,(LP Ag)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
二胺氧化酶(DAO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diamine Oxidase (DAO)48T/96TY10085Rattus norvegicus (Rat)
高鐵血紅蛋白還原酶(MHBR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methemoglobin Reductase (MHBR)48T/96TY10086Mus musculus (Mouse)
高鐵血紅蛋白還原酶(MHBR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methemoglobin Reductase (MHBR)48T/96TY10087Rattus norvegicus (Rat)
環(huán)加氧酶2(PTGS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)48T/96TY10088Mus musculus (Mouse)
絲裂原激活蛋白激酶10(MAPK10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase 10 (MAPK10)48T/96TY10089Rattus norvegicus (Rat)
神經(jīng)調(diào)節(jié)素1(NRG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuregulin 1 (NRG1)48T/96TY10090Homo sapiens (Human)
表皮生長因子受體2(EGFR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Epidermal Growth Factor Receptor 2 (EGFR2)48T/96TY10091Homo sapiens (Human)
金屬硫蛋白2(MT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metallothionein 2 (MT2)48T/96TY10092Homo sapiens (Human)
微管蛋白β(TUBβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tubulin Beta (TUBb)48T/96TY10093Homo sapiens (Human)
G組著色性干皮病偶聯(lián)因子(XPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Xeroderma Pigmentosum, Complementation Group G (XPG)48T/96TY10094Homo sapiens (Human)
乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactate Dehydrogenase (LDH)48T/96TY10095Homo sapiens (Human)
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY10096Canis familiaris; Canine (Dog)
CD3d分子(CD3d)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 3d (CD3d)48T/96TY10097Mus musculus (Mouse)
CD19分子(CD19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 19 (CD19)48T/96TY10098Mus musculus (Mouse)
癌胚抗原(CEA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen (CEA)48T/96TY10099Mus musculus (Mouse)
CD3d分子(CD3d)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 3d (CD3d)48T/96TY10100Rattus norvegicus (Rat)
凝血因子Ⅶ(F7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor VII (F7)48T/96TY10101Homo sapiens (Human)
角蛋白13(KRT13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 13 (KRT13)48T/96TY10102Homo sapiens (Human)
白介素5(IL5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY10103Sus scrofa; Porcine (Pig)
高遷移率族核小體結(jié)合域蛋白1(HMGN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1)48T/96TY10104Homo sapiens (Human)
叉頭框蛋白P3(FOXP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein P3 (FOXP3)48T/96TY10105Homo sapiens (Human)
乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactate Dehydrogenase (LDH)48T/96TY10106Mus musculus (Mouse)
白介素1受體關(guān)聯(lián)激酶2(IRAK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 2 (IRAK2)48T/96TY10107Mus musculus (Mouse)
白介素1受體關(guān)聯(lián)激酶2(IRAK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Associated Kinase 2 (IRAK2)48T/96TY10108Rattus norvegicus (Rat)
小核糖核蛋白多肽D1(SNRPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1)48T/96TY10109Mus musculus (Mouse)
神經(jīng)絲蛋白3(NEF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurofilament 3 (NEF3)48T/96TY10110Mus musculus (Mouse)
Apelin 36蛋白(AP36)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apelin 36 (AP36)48T/96TY10111Homo sapiens (Human)
載脂蛋白C3(APOC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein C3 (APOC3)48T/96TY10112Homo sapiens (Human)
白介素7(IL7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 7 (IL7)48T/96TY10113Rattus norvegicus (Rat)
AMP激活蛋白激酶β1(PRKAβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase, AMP Activated Beta 1 (PRKAb1)48T/96TY10114Homo sapiens (Human)
白介素6受體(IL6R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 Receptor (IL6R)48T/96TY10115Homo sapiens (Human)
丙酮酸脫氫酶激酶同工酶4(PDK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)48T/96TY10116Rattus norvegicus (Rat)
丙酮酸脫氫酶激酶同工酶4(PDK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)48T/96TY10117Mus musculus (Mouse)
胞漿型磷脂酶A2(cPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Cytosolic (cPLA2)48T/96TY10118Rattus norvegicus (Rat)
ⅡD組磷脂酶A2(PLA2G2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Group IID (PLA2G2D)48T/96TY10119Rattus norvegicus (Rat)
三葉因子3(TFF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 3, Intestinal (TFF3)48T/96TY10120Homo sapiens (Human)
防御素β1(DEFβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Defensin Beta 1 (DEFb1)48T/96TY10121Mus musculus (Mouse)
凝血因子Ⅷ(F8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor VIII (F8)48T/96TY10122Homo sapiens (Human)
載脂蛋白F(APOF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein F (APOF)48T/96TY10123Homo sapiens (Human)
載脂蛋白F(APOF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein F (APOF)48T/96TY10124Mus musculus (Mouse)
磷脂酰絲氨酸(PS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidylserine (PS)48T/96TY10125Homo sapiens (Human)
磷脂酰絲氨酸(PS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidylserine (PS)48T/96TY10126Mus musculus (Mouse)
成纖維細(xì)胞生長因子10(FGF10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 10 (FGF10)48T/96TY10127Rattus norvegicus (Rat)
白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY10128Rhesus monkey (Simian)
白介素4(IL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY10129Rhesus monkey (Simian)
載脂蛋白B48(APOB48)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein B48 (APOB48)48T/96TY10130Homo sapiens (Human)
受磷蛋白(PLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholamban (PLN)48T/96TY10131Homo sapiens (Human)
真核翻譯起始因子3A(EIF3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A)48T/96TY10132Homo sapiens (Human)
43kDa膠原凝集素(CL43)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collectin Of 43kDa (CL43)48T/96TY10133Bos taurus; Bovine (Cattle)
血管緊張素轉(zhuǎn)化酶2(ACE2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)48T/96TY10134Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。